Methotrexate restores effector T cell responsiveness in juvenile idiopathic arthritis
نویسندگان
چکیده
Methods Peripheral blood mononuclear cells (PBMCs) of 25 JIA patients were isolated prior to the start of MTX (T0) and 3 (T3) and 6 (T6) months after MTX start. Frequency and phenotype of FoxP3+ Tregs were analyzed ex vivo by flow cytometry and their suppressive function in CFSE suppression assays. Proliferation of CD4+ and CD8+ Teffs was determined with CFSE upon a 4-day culture in the presence of anti-CD3. Teffs cytokine production was measured ex vivo by flow cytometry upon PMA/ionomycin stimulation and in culture supernatants with luminex.
منابع مشابه
PReS-FINAL-2100: Methotrexate treatment affects effector, but not regulatory T cells in juvenile idiopathic arthritis
Methods Peripheral blood samples were obtained from JIA patients at MTX start and 3 and 6 months thereafter. Treg numbers and phenotype were determined by flow cytometry and suppressive function in allogeneic suppression assays. Teff proliferation upon stimulation with anti-CD3, activation status and intracellular cytokine production were determined by flow cytometry. Effector cell responsivene...
متن کاملMethotrexate treatment affects effector but not regulatory T cells in juvenile idiopathic arthritis.
OBJECTIVE The balance between Treg and effector T cells (Teff) is crucial for immune regulation in JIA. How MTX, the cornerstone treatment in JIA, influences this balance in vivo is poorly elucidated. The aim of this study was to investigate quantitative and qualitative effects of MTX on Treg and Teff in JIA patients during MTX treatment. METHODS Peripheral blood samples were obtained from JI...
متن کاملMulticentric Carpotarsal Osteolysis Mimicking Juvenile Idiopathic Arthritis
Background Multicentric carpotarsal osteolysis (MCTO), a skeletal dysplasia presents in early childhood mimicking juvenile idiopathic arthritis (JIA). Recognition of this syndrome is essential to avoid unnecessary treatment with immunosuppressive agents because of different course and treatment. Case Report A 3-year-old boy presented with swelling and restriction of right wrist joint and left ...
متن کاملMethotrexate does not primarily affect Foxp3+ regulatory T cells in poly-articular juvenile idiopathic arthritis
Materials and methods We sampled 11 patients with active poly-articular JIA (poly-JIA) prior to and 3–6 months after initiating MTX. Moreover, 11 poly-JIA patients in remission on MTX were sampled prior to and 3–6 months after withdrawal of MTX. Frequency and characteristics of Foxp3+CD4+Treg and effector T cell subsets were analyzed by flowcytometry. Function of Treg was evaluated in suppressi...
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عنوان ژورنال:
دوره 9 شماره
صفحات -
تاریخ انتشار 2011